|
| Status |
Public on Apr 17, 2024 |
| Title |
GlulΔlyz2, liver injury 2 |
| Sample type |
SRA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
genotype: Gluldlyz2
treatment: Acute liver injury induction by LPS/D-GalN injection
|
| Treatment protocol |
Acute liver injury was induced by intraperitoneal injection of 1 mg/kg LPS and 200 mg/kg D-GalN
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from liver tissues by TRIzol (TaKaRa, 9109).
The libraries were prepared by the NEBNext Ultra RNA Library Prep Kit and performed on Illumina NovaSeq 6000 platform in Mingma Technologies Co., Ltd (Shanghai, China).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
KOLD4
|
| Data processing |
Base calling was performed with RTAv2.7.6, and the fastq files were generated by bcl2fastqv 2.15.0 (Illumina,Inc.).
Alignment of the trimmed RNA-seq reads to the ensemble mouse genome assembly (GRCm39 (GCA_000001635.9)
The counts of the reads mapped to individual genes were calculated by feature Countsv 2.0.3.
DEGs were identified by the DESeq2 (version3.15; https://bioconductor.org/packages/DESeq2/), a cut-off of padj<0.05 and |log2(foldchange)|>1 was applied.
Assembly: GRCm39
Supplementary files format and content: Excel file includes FPKM values for all samples
|
| |
|
| Submission date |
Apr 16, 2023 |
| Last update date |
Apr 17, 2024 |
| Contact name |
Menglin Shi |
| E-mail(s) |
20211220003@fudan.edu.cn
|
| Organization name |
Fudan university
|
| Street address |
Dongan Road 130
|
| City |
Shanghai |
| ZIP/Postal code |
200000 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE229818 |
Meyoid-specific deficiency of Glutamine Synthetase Aggravates LPS/D-GalN-induced liver injury via the activation of monocyte-derived macrophages |
|
| Relations |
| BioSample |
SAMN34207914 |
| SRA |
SRX19982599 |
