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Status
Public on Sep 30, 2012
Title
WT
Sample type
RNA
Source name
wild type mouse
Organism
Mus musculus Characteristics
background strain: C57BL/6J
Biomaterial provider
Jackson
Growth protocol
Cells were grown in 25 ml flasks in the presence of DMEM plus 20% fetal bovine serum in the presence of penicillin, streptomycin, and amphotericin B until nearly confluent.
Extracted molecule
total RNA
Extraction protocol
RNA was extracted using Trizol according to the manufacturer's recommendations, followed by additional purification by the RNeasy procedure (Qiagen).
Label
biotin
Label protocol
Labeling was carried out by SABiosciences according to their protocols for the oligo array EMM405, by synthesizing labeled cRNA. See http://www.sabiosciences.com/manuals/DNAMicroarrays.pdf
Hybridization protocol
cRNAs were hybridized to 60-mer probes attached to nylon membrane, washed, and viewed for signal as described in the manual (see label protocol).
Scan protocol
See the manual described under label protocol:
Description
hind limb muscle tissue subjected to preplating procedure to isolate muscle derived stem cells
Data processing
GEArray Expression Analysis Suite (SuperArray BioScience Corp., Frederick, MD)
Submission date
Apr 29, 2011
Last update date
Sep 30, 2012
Contact name
Robert Gelfand
Organization name
LABiomed at Harbor UCLA, and Charles Drew Univ
Department
surgery
Lab
Gonzalez-Cadavid
Street address
1124 W. Carson St
City
Torrance
State/province
CA
ZIP/Postal code
90502
Country
USA
Platform ID
GPL13474 Series (1)
GSE28986 Myostatin inactivation effects on myogenesis in vitro and in vivo
Data table header descriptions ID_REF VALUE Each value for WT, KO, MDX, and OCT4 is the software computed normalized intensity.
Data table ID_REF VALUE 1
116.79
2
110.07
3
0.73
4
0.10
5
4.48
6
39.94
7
5.20
8
19.92
9
0.39
10
88.35
11
92.85
12
87.27
13
110.71
14
1.14
15
6.38
16
0.13
17
1.15
18
0.65
19
0.70
20
0.42
288 2 Kbytes .Supplementary data files not provided Processed data included within Sample table
