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Sample GSM7181537 Query DataSets for GSM7181537
Status Public on Apr 18, 2024
Title Group A CIN3
Sample type RNA
Source name Serum collected from the patient
Organism Homo sapiens
Characteristics tissue: Pooled serum from 23 patients with CIN3
Extracted molecule total RNA
Extraction protocol RNA samples were extracted from cells using miRNesy mini kit (Qiagen).
Label Cy5
Label protocol Extracted total RNA was labeled with Cy5 using the 3D-Gene miRNA labeling kit (Toray, Kamakura, Japan)
Hybridization protocol Hybridized for 16 h at 32 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
Scan protocol 3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
Description A total of 2 micro gram of extracted total RNA from the pooled samples in each disease category was prepared and 250 ng of this was labelled with a 3D-Gene miRNA labelling kit (Toray) followed by hybridizing onto 3D-Gene Human miRNA Oligo chips (Toray).
3 CIN3
Data processing The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
Submission date Apr 18, 2023
Last update date Apr 18, 2024
Contact name Satoshi Kondo
Organization name Toray Industries,Inc.
Street address 10-1 tebiro 6-chome
City Kamakura
State/province Kanagawa
ZIP/Postal code 248-8555
Country Japan
Platform ID GPL21263
Series (1)
GSE229935 microRNA profile in the serum of the cervical neoplasia

Data table header descriptions
VALUE Normalized signal intensity

Data table
MIMAT0000062 69.829963
MIMAT0000063 83.646108
MIMAT0000064 64.01314
MIMAT0000065 58.249452
MIMAT0000066 10.649959
MIMAT0000067 17.559637
MIMAT0000068 96.469103
MIMAT0000069 598.506308
MIMAT0000070 121.390729
MIMAT0000071 14.178131
MIMAT0000072 13.555261
MIMAT0000073 24.000934
MIMAT0000074 150.817342
MIMAT0000075 96.171978
MIMAT0000076 47.858487
MIMAT0000077 123.605664
MIMAT0000078 184.949634
MIMAT0000080 160.690758
MIMAT0000081 117.813173

Total number of rows: 2565

Table truncated, full table size 50 Kbytes.

Supplementary file Size Download File type/resource
GSM7181537_SH57664_Cell2.txt.gz 62.8 Kb (ftp)(http) TXT
Processed data included within Sample table

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