|
| Status |
Public on Apr 18, 2024 |
| Title |
Group A CIN3 |
| Sample type |
RNA |
| |
|
| Source name |
Serum collected from the patient
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Pooled serum from 23 patients with CIN3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using miRNesy mini kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Extracted total RNA was labeled with Cy5 using the 3D-Gene miRNA labeling kit (Toray, Kamakura, Japan)
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 32 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
A total of 2 micro gram of extracted total RNA from the pooled samples in each disease category was prepared and 250 ng of this was labelled with a 3D-Gene miRNA labelling kit (Toray) followed by hybridizing onto 3D-Gene Human miRNA Oligo chips (Toray).
3 CIN3
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Apr 18, 2023 |
| Last update date |
Apr 18, 2024 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Street address |
10-1 tebiro 6-chome
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21263 |
| Series (1) |
| GSE229935 |
microRNA profile in the serum of the cervical neoplasia |
|
