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Status |
Public on Jul 14, 2023 |
Title |
Fetal-MSC-1 |
Sample type |
SRA |
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Source name |
mesenchymal stem cells
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Organism |
Homo sapiens |
Characteristics |
cell type: mesenchymal stem cells treatment: non differentiation time: Day 0
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen). 500 ng of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using LEXOGEN Quant-Seq Library Prep Kit (Cat#001.24) standard protocols.
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
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Data processing |
Illumina Casava1.8 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence(using fastx_trimmer), then mapped to hg19 (UCSC) whole genome using Bowtie2 Read count extraction using bedtools. Assembly: hg19 Supplementary files format and content: tab-delimited text files include read count values for each Sample
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Submission date |
Apr 18, 2023 |
Last update date |
Jul 14, 2023 |
Contact name |
Hyun-Ju An |
E-mail(s) |
yks486ahj@naver.com
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Organization name |
CHA university
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Street address |
Seongnam-si, 13496
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City |
Seongnam |
State/province |
--- Select One --- |
ZIP/Postal code |
13496 |
Country |
South Korea |
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Platform ID |
GPL18573 |
Series (1) |
GSE229945 |
Fetal mesenchymal stem cells promote osteogenesis via Notch signaling |
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Relations |
BioSample |
SAMN34232890 |
SRA |
SRX20000332 |
Supplementary file |
Size |
Download |
File type/resource |
GSM7181675_Fetal-MSC-1.txt.gz |
121.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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