|
Status |
Public on May 17, 2012 |
Title |
LU143 |
Sample type |
RNA |
|
|
Source name |
Lung tumor, adenocarcinoma, smoker
|
Organism |
Homo sapiens |
Characteristics |
tissue: lung carcinoma assay: LU143 osbin: 1 os: 1.254794521 age: 71 Sex: M gexcluster_ac: AC1 smoking: smoker tnm: T3N0M0 Stage: IIB ckit_ihc: 0 her2_ihc: 1 pakt_ihc: 0 pten_ihc: 1 egfr_ihc: 0 egfr_amp: 0 egfr_mut: 0 pik3ca_mut: 0 kras_mut: 0 histology: AC
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from fresh frozen lung tumor tissue was extracted as described PMID:17334996. RNA concentrations were measured using a Nanodrop (NanoDrop Technologies). RNA integrity was determined by an Agilent Bioanalyzer.
|
Label |
biotin
|
Label protocol |
Illumina®Totalprep-96RNA Amplification kit 4397949 RevB
|
|
|
Hybridization protocol |
Illumina hybridization protocol (Manuell 11322187 Revision A)
|
Scan protocol |
Standard Illumina iScan scanning protocol Scan setting file: Direct Hyb
|
Data processing |
The data were normalised using quantile normalisation implemented in BASE, http://base.thep.lu.se/
|
|
|
Submission date |
May 02, 2011 |
Last update date |
Oct 09, 2012 |
Contact name |
Johan Staaf |
Organization name |
SCIBLU - Swegene Centre for Integrative Biology at Lund University
|
Street address |
Medicon Village
|
City |
Lund |
ZIP/Postal code |
SE-223 81 |
Country |
Sweden |
|
|
Platform ID |
GPL6947 |
Series (2) |
GSE29016 |
Relation between smoking history and gene expression profiles in lung adenocarcinomas |
GSE29066 |
Molecular profiling of primary lung carcinomas |
|