|
| Status |
Public on Jul 27, 2011 |
| Title |
hybrid_FlowerBud_RNA1 |
| Sample type |
RNA |
| |
|
| Source name |
flower bud
|
| Organism |
Arabidopsis thaliana x Arabidopsis lyrata |
| Characteristics |
ecotype: Be-0 x LF10
tissue: flower bud
|
| Treatment protocol |
3 four-weeks-old leaves, 3-4 open flowers and 3-4 flower buds
|
| Growth protocol |
stratifying for 7 days at 4°C and growing in the greenhouse under 16h cool white light supplemented with red light at 20°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Plant Mini Kit
|
| Label |
Biotin
|
| Label protocol |
Biotin, Invitrogen Bioprime random labeling
|
| |
|
| Hybridization protocol |
Standard hybridization protocol for tiling array from Affymetrix
|
| Scan protocol |
Affymetrix Scanner 7G
|
| Data processing |
GeneChip Operating Software Version 1.4 (GCOS)
primary_data_description: raw intensity was read from .CEL file, log transformed, background corrected, quantile normalzied, then convert back to normal scale
|
| |
|
| Submission date |
May 03, 2011 |
| Last update date |
Aug 01, 2011 |
| Contact name |
Fei He |
| E-mail(s) |
fei.he@uni-muenster.de
|
| Organization name |
Institute for Evolution and Biodiversity
|
| Department |
Münster University
|
| Lab |
de Meaux
|
| Street address |
Hüfferstr. 1
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13483 |
| Series (1) |
| GSE29037 |
Allele-specific expression between Arabidopsis genus |
|
