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Status |
Public on May 29, 2012 |
Title |
Runx1-/VE-cadherin+/Flk1+, expression |
Sample type |
RNA |
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Source name |
FACS-sorted day 6 differentiated ES cells, Runx1-/VE-cadherin+/Flk1+
|
Organism |
Mus musculus |
Characteristics |
cell line: Runx1(Venus/+) ES cell line cell type: differentiated ES cells days of differentiation: 6 cell subpopulation: Runx1-/VE-cadherin+/Flk1+
|
Treatment protocol |
No treatment beyond the normal growth protocol was used.
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Growth protocol |
1 x 10^5 undifferentiated ES cells were cultured on confluent OP9 cell layers in 10-cm dishes to induce differentiation. After 6 days of ES cell differentiation, cultured cells were harvested for FACS analysis and sorting.
|
Extracted molecule |
total RNA |
Extraction protocol |
Extraction of total RNA was performed by using the QIAGEN RNeasy Micro kit according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
The 3'IVT Express Kit was used for biotin labeling according to the manufacturer's instructions (Affymetrix).
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Hybridization protocol |
RNA was subsequently fragmented and hybridized to the GeneChip according to the manufacturer’s instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix).
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Description |
Gene expression data from FACs-sorted day 6 differentiated ES cells.
Runx1(Venus/+) ES cell line: The Venus gene was placed in the P2-5'UTR under the control of the Runx1 proximal promoter P2. Parental ES cells are CCE ES cells.
|
Data processing |
The data were processed using MAS5.0 (Affymetrix GCOS Ver.1.3).
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Submission date |
May 06, 2011 |
Last update date |
May 29, 2012 |
Contact name |
Anagha M Joshi |
E-mail(s) |
aj379@cam.ac.uk
|
Phone |
+44 01223 336822
|
Organization name |
University of cambridge
|
Department |
Dept. of haematology, CIMR
|
Lab |
Dr. Gottgens
|
Street address |
Wellcome trust/MRC building, Hills road
|
City |
Cambridge |
ZIP/Postal code |
CB20XY |
Country |
United Kingdom |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE29112 |
The transcriptional program controlled by Runx1 during early hematopoietic development (expression data) |
GSE29515 |
The transcriptional program controlled by Runx1 during early hematopoietic development |
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