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Sample GSM7211472 Query DataSets for GSM7211472
Status Public on Apr 30, 2023
Title Young hamster lung number 4
Sample type RNA
 
Source name lung tissue
Organism Mesocricetus auratus
Characteristics age: 2 months old
tissue: lung
Treatment protocol No specific treatment - generation of young and old cohorts
Growth protocol Golden Hamster of 2 and 22 months were used
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from frozen lungs after using Nucleospin RNA kit (Macherey Nagel)
Label Cy3
Label protocol cRNAs were synthesized and labeled with the Cy3 dye from 100 ng of total RNA using the one-color Low Input Quick Amp Labeling Kit (Agilent Technologies) following the manufacturer's recommandations, followed by RNeasy Mini column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
 
Hybridization protocol 1.5 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. After fragmentation 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent GE Whole Human Genome Oligo Microarrays (Agilent-026652) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried briefly in acetonitrile.
Scan protocol Microarrays were scanned with an Agilent DNA microarray scanner G2565CA (Agilent Technologies).Fluorescent signals were extracted and normalized with the Feature Extraction software version 10.5.1.1 (Agilent Technologies),
Data processing The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities.
 
Submission date Apr 21, 2023
Last update date May 01, 2023
Contact name jean-michel flaman
E-mail(s) jean-michel.flaman@lyon.unicancer.fr
Phone +33687477812
Organization name Inserm 1052
Department CRCL
Street address 28 rue Laennec
City Lyon
ZIP/Postal code 69373
Country France
 
Platform ID GPL33354
Series (1)
GSE230301 Transcriptome profiling of lungs from young and aged hamster

Data table header descriptions
ID_REF
VALUE Log2 relative expression after 75th percentile intensity signal normalization and baseline adjusted on the mean of all samples using GeneSpring GX 12.6 software (Agilent Technologies)

Data table
ID_REF VALUE
CUST_1855_PI448782077 -0.003917694
CUST_17556_PI448782077 0.05113077
CUST_19643_PI448782077 0.102175474
CUST_2016_PI448782077 -0.09615719
CUST_5153_PI448782077 -0.53872705
CUST_9494_PI448782077 0.3076558
CUST_12469_PI448782077 0.020744458
CUST_23271_PI448782077 -1.2663198
CUST_25438_PI448782077 0.01942885
CUST_852_PI448782077 -0.035330653
CUST_15215_PI448782077 -0.030242443
CUST_1590_PI448782077 0.0023469925
CUST_19840_PI448782077 0.18552387
CUST_5965_PI448782077 0.64331675
CUST_11154_PI448782077 -0.11429548
CUST_9371_PI448782077 -0.2667737
CUST_825_PI448782077 -0.14194083
CUST_13629_PI448782077 0.121925354
CUST_22731_PI448782077
CUST_205_PI448782077 -0.15819263

Total number of rows: 18088

Table truncated, full table size 599 Kbytes.




Supplementary file Size Download File type/resource
GSM7211472_SG12034169_258641410006_S001_GE1_1105_Oct12_1_1.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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