|
| Status |
Public on Apr 18, 2024 |
| Title |
P17OIR+ISOX DUAL_1 |
| Sample type |
RNA |
| |
|
| Source name |
retina, oxygen-induced retinopathy(OIR), Inhibitor (ISOX DUAL)
|
| Organism |
Mus musculus |
| Characteristics |
tissue: retina
strain: C57BL/6JJmsSlc
condition: oxygen-induced retinopathy(OIR), Inhibitor (ISOX DUAL)
|
| Treatment protocol |
Vitreous injection was performed immediately after the end of hyperoxia.
|
| Growth protocol |
oxygen-induced retinopathy(OIR) Seven-day-old mice were grown in 75% oxygen for 5 days and then housed in air.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 45° for 5 minutes centrifuge at max speed for 1 minute. • Transfer 130μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 16 hours at 45° at 60rpm • Fluidics washing is FS450_0002
|
| Scan protocol |
Gene ChIP Scanner 3000 7G System
|
| Description |
SAMPLE 1
|
| Data processing |
Data were processed using R. MAT background correction were applied to probe intensities, gene level expression were summarized from the corrected intensities and then quantile normalized.
|
| |
|
| Submission date |
Apr 26, 2023 |
| Last update date |
Apr 18, 2024 |
| Contact name |
Kengo Nakanishi |
| E-mail(s) |
kengo24195@gmail.com
|
| Phone |
09012288003
|
| Organization name |
University of Fukui
|
| Department |
Applied Chemistry & Biotechnology
|
| Street address |
3-9-1 Bunkyo,Fukui
|
| City |
Fukui |
| ZIP/Postal code |
9100017 |
| Country |
Japan |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE230668 |
Effects of inhibitors in mice with oxygen-induced retinopathy |
|
