|
| Status |
Public on Nov 29, 2011 |
| Title |
SVZ cells_MCAo3_7d |
| Sample type |
other |
| |
|
| Source name |
total RNA from MCAo SVZ neural progenitor cells
|
| Organism |
Rattus norvegicus |
| Characteristics |
age: 3-4 months
cells: cultured neural progenitor cells in subventricular zone of the brain
treatment: middle cerebral artery occlusion
|
| Extracted molecule |
other |
| Extraction protocol |
miRNeasy Mini Kit
|
| Label |
biotin
|
| Label protocol |
The RNA samples were 3’-end labeled with biotin dye using the FlashTagTM Biotin RNA Labeling Kits
|
| |
|
| Hybridization protocol |
Labeled LMW RNA samples were hybridized to the MicroRNA microarrays according to conditions recommended in the Flash Taq RNA labeling Kit manual.
|
| Scan protocol |
The microarrays were scanned on an Axon Genepix 4000B scanner (Affymetrix Inc),
|
| Description |
Gene expression data from ischemic cultured SVZ neural progenitor cells
|
| Data processing |
Data were extracted from images using GenePix V4.1 software. miRNA QC Tool (Affymetrix Inc), a software for data summarization, Log2 transformation, normalization and quality control, was used.
|
| |
|
| Submission date |
May 13, 2011 |
| Last update date |
Nov 29, 2011 |
| Contact name |
Xian Shuang Liu |
| E-mail(s) |
xsliu@neuro.hfh.edu
|
| Organization name |
Henry Ford Hospital
|
| Street address |
2799 West Grand BLVD
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48202 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE29287 |
MicroRNA profiling in subventricular zone after stroke: miR-124a regulates proliferation of neural progenitor cells through Notch signaling pathway |
|
