|
Status |
Public on Oct 14, 2024 |
Title |
Arg_cntl3 |
Sample type |
RNA |
|
|
Source name |
OT-1 cells activated in control culture medium without 5 ng/ml TGF-b stimulation for 2.5 days
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 cell line: OT-1 treatment: activated in control culture medium without 5 ng/ml TGF-b stimulation for 2.5 days
|
Treatment protocol |
Sorted cells were lysed with lysis buffer from Rneasy Micro kit from Qiagen.
|
Growth protocol |
OT-1 cells were activated in control or arginine-deficient culture medium with or without stimulation of 5 ng/ml TGF-b for 2.5 days
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA samples were prepared according to the prototol of Rneasy Micro kit from Qiagen,
|
Label |
biotin
|
Label protocol |
High-quality RNA was processed using Thermo Fisher (Affymetrix) Whole Transcript (WT) Plus assay kit
|
|
|
Hybridization protocol |
Labeled cDNA was included in a hybridization mix that was incubated on the Clariom S mouse array for 16 hours at 45◦c while rotating at 60 rpm.
|
Scan protocol |
GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
|
Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console software v1.1
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|
|
Submission date |
Apr 30, 2023 |
Last update date |
Oct 14, 2024 |
Contact name |
Hongbo Chi |
E-mail(s) |
hongbo.chi@stjude.org
|
Organization name |
St Jude Children's Research Hospital
|
Department |
Immunology
|
Street address |
262 Danny Thomas Place
|
City |
Memphis |
State/province |
TN |
ZIP/Postal code |
38105 |
Country |
USA |
|
|
Platform ID |
GPL23038 |
Series (2) |
GSE231342 |
Interplay between nutrient stress and lysosomal signaling imprints immune memory in tissues [microarray4] |
GSE231502 |
CRISPR screens unveil nutrient-dependent lysosomal and mitochondrial nodes impacting intestinal tissue-resident memory CD8+ T cell formation |
|