High capacity cDNA reverse transcriptions protocol, Applied Biosystems
Hybridization protocol
n/a
Scan protocol
n/a
Description
Disease Control 1 Plate 2 Roche Lightcycler
Data processing
Matrix non-normalized was generated by calculating mean of CT values for each gene including housekeeping gene, GAPDH, for all the patients with SARS-CoV2 as well as all the disease controls recruited in the study. Matrix normalized was generated by subtratcing the mean Ct value of GAPDH from the gene of interest (GOI). This yielded two normalized integers set, (mean CT GOI in patients with SARS-CoV2 -mean CT housekeeping gene, GAPDH, in patients with SARS-CoV2) and (Mean Ct GOI disease controls - mean Ct housekeeping gene, GAPDH, in disease controls). These normalised values were referred to as delta Ct values. Delta delta Ct values for each GOI were calculated by subtracting delta ct in SARS-CoV2 patient - delta Ct value in disease controls. Fold change of GOI was calculated by 2 (to the power delta delta Ct) for each gene.
Submission date
May 02, 2023
Last update date
Apr 17, 2024
Contact name
Vikas Agarwal
Organization name
Sanjay Gandhi Postgraduate Institute of Medical Sciences
