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Status |
Public on Sep 01, 2005 |
Title |
CPGSE#4, chip MOE430B |
Sample type |
RNA |
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Source name |
haematopoeitic stem cells, bone marrow
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Organism |
Mus musculus |
Characteristics |
Strain: C57BL/6 Gender: Female Defining markers: CD45+ScaI+
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Extracted molecule |
total RNA |
Extraction protocol |
RNA prep method: Qiagen Cell purification method: Moflo cell sorter Method for estimating purity: cell sorter reading
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Label |
Biotin
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Description |
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis. Stembase Experiment ID: E191 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=191 SCGP Sample ID: S147 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=191#SAMPLE_109 Short description: Mouse bone marrow stained with CD45-APC & Scal-PE, sorted on Moflo for +/+ cells Analysis requested: microarray Estimated purity: 95-98% RNA concentration: 50ng/ul Num cells for RNA prep: 6.9 X 10^5 Sample volume: 10ul
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Data processing |
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
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Submission date |
Aug 31, 2005 |
Last update date |
Jan 19, 2006 |
Organization |
Ottawa Hospital Research Institute |
Phone |
(613) 737-8899 -73255
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Department |
Cellular and Molecular Medicine
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Lab |
Ottawa Bioinformatics Core Facility
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Street address |
501 Smyth Rd.
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City |
Ottawa |
State/province |
ON |
ZIP/Postal code |
K1H 8L6 |
Country |
Canada |
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Platform ID |
GPL340 |
Series (1) |
GSE3241 |
Sca1-CD45 sorted bone marrow |
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Data table header descriptions |
ID_REF |
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VALUE |
'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06. |
ABS_CALL |
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC) |
DETECTION P-VALUE |
'detection p-value', p-value that indicates the significance level of the detection call |
Data table |
ID_REF |
VALUE |
ABS_CALL |
DETECTION P-VALUE |
AFFX-BioB-5_at |
8328.82 |
P |
0.00401721 |
AFFX-BioB-M_at |
11752.6 |
P |
0.000126798 |
AFFX-BioB-3_at |
7666.83 |
P |
9.4506e-05 |
AFFX-BioC-5_at |
19200.2 |
P |
0.000146581 |
AFFX-BioC-3_at |
14876.9 |
P |
0.00010954 |
AFFX-BioDn-5_at |
17223 |
P |
0.000195116 |
AFFX-BioDn-3_at |
88015 |
P |
5.16732e-05 |
AFFX-CreX-5_at |
163551 |
P |
4.42873e-05 |
AFFX-CreX-3_at |
282063 |
P |
4.42873e-05 |
AFFX-DapX-5_at |
177953 |
P |
4.42873e-05 |
AFFX-DapX-M_at |
406303 |
P |
4.42873e-05 |
AFFX-DapX-3_at |
444188 |
P |
4.42873e-05 |
AFFX-LysX-5_at |
215936 |
P |
4.42873e-05 |
AFFX-LysX-M_at |
436926 |
P |
4.42873e-05 |
AFFX-LysX-3_at |
403663 |
P |
4.42873e-05 |
AFFX-PheX-5_at |
75790.9 |
P |
4.42873e-05 |
AFFX-PheX-M_at |
224309 |
P |
4.42873e-05 |
AFFX-PheX-3_at |
428173 |
P |
4.42873e-05 |
AFFX-ThrX-5_at |
11230 |
P |
0.000126798 |
AFFX-ThrX-M_at |
60862.5 |
P |
4.42873e-05 |
Total number of rows: 22575
Table truncated, full table size 666 Kbytes.
Supplementary file |
Size |
Download |
File type/resource |
GSM72979.CEL.gz |
3.0 Mb |
(ftp)(http) |
CEL |
GSM72979.EXP.gz |
494 b |
(ftp)(http) |
EXP |
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