|
| Status |
Public on Jul 01, 2011 |
| Title |
Input 1 |
| Sample type |
SRA |
| |
|
| Source name |
IB4 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: IB4
cell type: EBV immotalized lymphocyte
antibody: None
|
| Growth protocol |
IB4 LCLs,were grown in RPMI media (Invitrogen) supplemented with 10% fetal plex (Gemini), glutamine. The cells were maintained at 3x105/ml
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
IB4 cells were cross-linked with 1% Formaldehyde at 370C for 10 minutes. Cell DNA was sonicated to an average size of ~500bp. Rabbit anti EBNA2 or RBPJ were incubated with lysates overnight at 40C. Salmon sperm DNA protein A agarose beads (Millipore) were added to the lysate and incubated at 40C for 1 hour. After extensive wash, DNA was eluted from the agarose beads, crossliking reversed at 650C for 4 hours, and DNA further purified using PCR columns (Qiagen). ChIP-seq DNA sample preparation kits were used to prepared ChIP-seq libraries (Illumina).
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Lymphoblastoid cell line
Input
|
| Data processing |
Allignment: Sequence reads were mapped to hg.18 using Illumina Genome Analyzer Pipeline allowing 2 mismatches
|
| |
|
| Submission date |
May 24, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
James Zou |
| E-mail(s) |
jzou@fas.harvard.edu
|
| Phone |
6173010832
|
| Organization name |
Harvard University
|
| Street address |
185 Cambridge St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL9052 |
| Series (1) |
| GSE29498 |
Epstein-Barr Virus Exploits Intrinsic B-Lymphocyte Transcription Programs to Achieve Immortal Cell Growth |
|
| Relations |
| SRA |
SRX092454 |
| BioSample |
SAMN00710401 |
