|
Status |
Public on Apr 24, 2024 |
Title |
M1-AGDT c32 AG |
Sample type |
SRA |
|
|
Source name |
585B1(XY) AGDT7453
|
Organism |
Homo sapiens |
Characteristics |
cell line: 585B1-7453 cell type: differentiated hPGCLC transgene: TFAP2C-EGFP, DAZL-tdTomato knock-in genotype: WT time point: culture day 32 treatment: Expansion culture with BMP signalling
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Gemomic DNA extraction was integrated in "library construction protocol" process. EM-seq libraries were generated as described previously (Gyobu-Motani, 2023). EM-seq, (Vaisvila, 2021)
|
|
|
Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
M1-AGDT (585B1-7453) expansion culture c32 BMP2(+) AG(+) DT(-)
|
Data processing |
Paired-end reads were processed with Trim_Galore! V0.6.3 and mapped on the GRCh38.p12 genome using Bismark v0.22.1 and Bowtie2 v2.3.4.1 with the “-X 2000” option. BAM files were, then, processed with deduplicate_bismark script to remove PCR duplicates, and bismark_methylation_extractor to count methyl- and unmehyl-Cytosine per at CpG or CpA sequences. Assembly: GRCh38.p12 Supplementary files format and content: bismark report file Library strategy: EM-seq
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|
|
Submission date |
May 05, 2023 |
Last update date |
Apr 24, 2024 |
Contact name |
Yukihiro Yabuta |
E-mail(s) |
yabyab@anat2.med.kyoto-u.ac.jp
|
Organization name |
Kyoto University, Graduate school of medicine
|
Department |
Anatomy and Cell Biology
|
Street address |
Yoshida-Konoe-cho, Sakyo-ku
|
City |
Kyoto |
State/province |
Kyoto |
ZIP/Postal code |
606-8501 |
Country |
Japan |
|
|
Platform ID |
GPL24676 |
Series (2) |
GSE231813 |
In vitro reconstitution of epigenetic reprogramming in the human germ line [EM-Seq] |
GSE232078 |
In vitro reconstitution of epigenetic reprogramming in the human germ line |
|
Relations |
BioSample |
SAMN34721817 |
SRA |
SRX20238389 |