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Sample GSM7310578 Query DataSets for GSM7310578
Status Public on Mar 28, 2024
Title C1498 hsc-lipo 2
Sample type SRA
 
Source name C1498
Organism Mus musculus
Characteristics cell line: C1498
cell type: Myeloid cell
genotype: WT
treatment: Ara-C@HSC-Lipo
Treatment protocol About 100 0,000 cells were inoculated in each hole of the flat bottom 6-well plate.Cells were harvested 24 hours after treatment with the drug.
Growth protocol Ka539 cells were mouse B-cell leukemia/lymphoma mixed cell line cultured in 45% DMEM +45% IMDM +10% FBS+β-Me medium. C1498, a mouse acute myeloid leukemia cell line, was cultured with DMEM+10% FBS and passed every 72 hours.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by trizol method, and RNA was extracted by isopropyl alcohol, and washed with 75% alcohol.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing Illumina Casava1.7 software used for basecalling.
Raw data were trimmed by Trimmomatic (v0.39).
Clean data were aligned to the mouse reference genome mm10 by HISAT2 (v2.1.0) with default setting.
htseq-count (v0.13.5) was used to calculate genes counts with parameter “--mode intersection-strict --stranded no --minaqual 1”.
Assembly: mm10
Supplementary files format and content: tab-delimited text files include raw counts for each Sample .
 
Submission date May 09, 2023
Last update date Mar 28, 2024
Contact name Li jinxin
E-mail(s) lijinxin1015@gmail.com
Organization name Zhejiang University
Street address 1369 West Wenyi Road
City Hangzhou
ZIP/Postal code 310058
Country China
 
Platform ID GPL17021
Series (1)
GSE232029 Hematopoietic stem cell membrane-coated vesicles for targeted drug delivery to the bone marrow and elimination of leukemia cells
Relations
BioSample SAMN35006726
SRA SRX20263162

Supplementary file Size Download File type/resource
GSM7310578_4.txt.gz 219.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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