|
| Status |
Public on Mar 28, 2024 |
| Title |
Ka539 hsc-lipo 2 |
| Sample type |
SRA |
| |
|
| Source name |
Ka539
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Ka539
cell type: lymphocyte
genotype: WT
treatment: Ara-C@HSC-Lipo
|
| Treatment protocol |
About 100 0,000 cells were inoculated in each hole of the flat bottom 6-well plate.Cells were harvested 24 hours after treatment with the drug.
|
| Growth protocol |
Ka539 cells were mouse B-cell leukemia/lymphoma mixed cell line cultured in 45% DMEM +45% IMDM +10% FBS+β-Me medium. C1498, a mouse acute myeloid leukemia cell line, was cultured with DMEM+10% FBS and passed every 72 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by trizol method, and RNA was extracted by isopropyl alcohol, and washed with 75% alcohol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Raw data were trimmed by Trimmomatic (v0.39).
Clean data were aligned to the mouse reference genome mm10 by HISAT2 (v2.1.0) with default setting.
htseq-count (v0.13.5) was used to calculate genes counts with parameter “--mode intersection-strict --stranded no --minaqual 1”.
Assembly: mm10
Supplementary files format and content: tab-delimited text files include raw counts for each Sample .
|
| |
|
| Submission date |
May 09, 2023 |
| Last update date |
Mar 28, 2024 |
| Contact name |
Li jinxin |
| E-mail(s) |
lijinxin1015@gmail.com
|
| Organization name |
Zhejiang University
|
| Street address |
1369 West Wenyi Road
|
| City |
Hangzhou |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE232029 |
Hematopoietic stem cell membrane-coated vesicles for targeted drug delivery to the bone marrow and elimination of leukemia cells |
|
| Relations |
| BioSample |
SAMN35006722 |
| SRA |
SRX20263166 |
