|
Status |
Public on Aug 29, 2024 |
Title |
BE2C_GFPsh-1 |
Sample type |
RNA |
|
|
Source name |
BE2C_GFPsh-1
|
Organism |
Homo sapiens |
Characteristics |
tumor cell line: Neuroblastoma
|
Treatment protocol |
BE(2)-C cells were infected with lentiviruses expressing shRNA to GFP (GFPsh) or human PUS7 (PUS7sh#33) for 6 days
|
Growth protocol |
BE(2)-C cells were cultured in DME/F-12 1:1 (HyClone SH30023) supplemented with 10% FBS (Fisher Scientific, FB12999102).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from the cell samples using Trizol (Invitrogen), and RNA quality and quantity was assessed using a Agilent BioAnalyzer and a Nanodrop Spectrophotometer.
|
Label |
biotin
|
Label protocol |
TdT-labled cRNA were prepared with an Affymetrix GeneChip WT Terminal labeling kit.
|
|
|
Hybridization protocol |
Standard Affymetrix hybridization protocol
|
Scan protocol |
Standard Affymetrix scanning protocol
|
Description |
RNA expression value
|
Data processing |
Data were normalized from CEL files using R/Bioconductor and gcRMA algorithm (http://homer.ucsd.edu/homer/basicTutorial/affymetrix.html).
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|
|
Submission date |
May 09, 2023 |
Last update date |
Aug 29, 2024 |
Contact name |
Han-Fei Ding |
E-mail(s) |
hding@uabmc.edu
|
Phone |
7067269419
|
Organization name |
University of Alabama at Birmingham
|
Department |
Pathology
|
Lab |
Ding
|
Street address |
WTI 602D, 1824 6th Avenue South
|
City |
Birmingham |
State/province |
Alabama |
ZIP/Postal code |
35294 |
Country |
USA |
|
|
Platform ID |
GPL16686 |
Series (1) |
GSE232058 |
Gene expression profiling of human neuroblastoma cell line BE(2)-C infected with lentiviruses expressing shRNA to GFP (GFPsh) or human PUS7 (PUS7sh#33) for 6 days |
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