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Sample GSM7311131 Query DataSets for GSM7311131
Status Public on Aug 29, 2024
Title BE2C_GFPsh-1
Sample type RNA
 
Source name BE2C_GFPsh-1
Organism Homo sapiens
Characteristics tumor cell line: Neuroblastoma
Treatment protocol BE(2)-C cells were infected with lentiviruses expressing shRNA to GFP (GFPsh) or human PUS7 (PUS7sh#33) for 6 days
Growth protocol BE(2)-C cells were cultured in DME/F-12 1:1 (HyClone SH30023) supplemented with 10% FBS (Fisher Scientific, FB12999102).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the cell samples using Trizol (Invitrogen), and RNA quality and quantity was assessed using a Agilent BioAnalyzer and a Nanodrop Spectrophotometer.
Label biotin
Label protocol TdT-labled cRNA were prepared with an Affymetrix GeneChip WT Terminal labeling kit.
 
Hybridization protocol Standard Affymetrix hybridization protocol
Scan protocol Standard Affymetrix scanning protocol
Description RNA expression value
Data processing Data were normalized from CEL files using R/Bioconductor and gcRMA algorithm (http://homer.ucsd.edu/homer/basicTutorial/affymetrix.html).
 
Submission date May 09, 2023
Last update date Aug 29, 2024
Contact name Han-Fei Ding
E-mail(s) hding@uabmc.edu
Phone 7067269419
Organization name University of Alabama at Birmingham
Department Pathology
Lab Ding
Street address WTI 602D, 1824 6th Avenue South
City Birmingham
State/province Alabama
ZIP/Postal code 35294
Country USA
 
Platform ID GPL16686
Series (1)
GSE232058 Gene expression profiling of human neuroblastoma cell line BE(2)-C infected with lentiviruses expressing shRNA to GFP (GFPsh) or human PUS7 (PUS7sh#33) for 6 days

Data table header descriptions
ID_REF
VALUE quantile normalized gene level expression values

Data table
ID_REF VALUE
16650001 1.64593
16650003 2.31021
16650005 2.81804
16650007 2.62902
16650009 0.63619
16650011 1.61421
16650013 4.79268
16650015 6.87779
16650017 4.32335
16650019 2.19077
16650021 3.55277
16650023 2.47319
16650025 4.7784
16650027 2.88435
16650029 4.42507
16650031 4.70098
16650033 1.6236
16650035 2.071
16650037 1.34168
16650041 4.97145

Total number of rows: 53617

Table truncated, full table size 884 Kbytes.




Supplementary file Size Download File type/resource
GSM7311131_BE2C_GFPsh-1_HuGene-2_0-st_.CEL.gz 7.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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