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Sample GSM731540 Query DataSets for GSM731540
Status Public on Jul 06, 2011
Title CUTLL1 DNMAML1 GSIwash2h-rep1
Sample type RNA
 
Source name CUTLL1 DNMAML1 GSIwash2h
Organism Homo sapiens
Characteristics cell line: CUTLL1 (T lymphoblastic leukemia / lymphoma)
Growth protocol CUTLL1 cells were cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal bovine serum, 2 mM L-glutamine, penicillin and streptomycin.
Extracted molecule total RNA
Extraction protocol Total RNA prepared with RNeasy Mini kit (Qiagen) was subjected to expression profiling in triplicate on Affymetrix human genome U133 plus 2.0 arrays.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7-10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Hybridization was performed according to standard protocols for Affymetrix HG-U133 plus 2.0 arrays.
Scan protocol Arrays were scanned using a GenePix 4000B microarray scanner.
Description Gene expression data from DN-MAML transduced CUTLL1 cells treated with compound E for 3days and washout compund E for 2 hours
Data processing Affymetrix expression data were normalized using the standard robust multichip average (RMA) package in R.
 
Submission date May 26, 2011
Last update date Jul 06, 2011
Contact name James Zou
E-mail(s) jzou@fas.harvard.edu
Phone 6173010832
Organization name Harvard University
Street address 185 Cambridge St.
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL570
Series (1)
GSE29544 Expression profiling of human T-LL cell line CUTLL1

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
1007_s_at 7.05
1053_at 8.09
117_at 5.8
121_at 5.83
1255_g_at 2.35
1294_at 5.43
1316_at 5.3
1320_at 4.96
1405_i_at 2.54
1431_at 3.23
1438_at 4.48
1487_at 6.27
1494_f_at 3.31
1552256_a_at 3.97
1552257_a_at 7.88
1552258_at 4.2
1552261_at 4.4
1552263_at 5.21
1552264_a_at 7.36
1552266_at 5.72

Total number of rows: 54675

Table truncated, full table size 840 Kbytes.




Supplementary file Size Download File type/resource
GSM731540_3114_12250_46_U133+2.0.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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