|
| Status |
Public on Jun 09, 2023 |
| Title |
X8a |
| Sample type |
SRA |
| |
|
| Source name |
K562 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: K562 cell line
cell type: lymphoblast cells
genotype: Cas9FCPF-sgRNA
treatment: JQ1-FCPF
time: 1h
|
| Treatment protocol |
Transfected with Cas9FCPF with/without sgRNA
1 µM JQ1 or 1 µM JQ1-FCPF
|
| Growth protocol |
K562 cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, Quality control and sequencing were performed by Azenta
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
RNA seq was performed by Azenta
|
| |
|
| Submission date |
May 10, 2023 |
| Last update date |
Jun 09, 2023 |
| Contact name |
Xinlai Cheng |
| E-mail(s) |
Cheng@pharmchem.uni-frankfurt.de
|
| Organization name |
Goethe university Frankfurt
|
| Department |
Pharmaceutical Chemistry
|
| Street address |
Max-von-Laue-Strasse 15. R. 3.652
|
| City |
Frankfurt |
| ZIP/Postal code |
D-60438 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE232132 |
Chem-CRISPR/dCas9 -a chemically induced platform for epigenome editing |
|
| Relations |
| BioSample |
SAMN35019518 |
| SRA |
SRX20275797 |
