|
Status |
Public on Mar 10, 2008 |
Title |
SAS1-HA vs WT. Replica 1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
untransformed plants
|
Organism |
Arabidopsis thaliana |
Characteristics |
Total RNA was extracted from 3 weeks old leaves of WT Arabidopsis thaliana Col-0
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from 3-4 weeks old rosettes using the RNAeasy plant mini kit (Qiagen) and subjected to reverse transcription and amplified by in vitro transcription with t7 polymerase (Ambion).
|
Label |
CY5
|
Label protocol |
Amplified RNA products were subjected to reverse transcription and then labeled with Cy3 and Cy5 by indirect amino-allyl method.
|
|
|
Channel 2 |
Source name |
SAS1-HA
|
Organism |
Arabidopsis thaliana |
Characteristics |
Total RNA was extracted from 3 weeks old leaves of SAS1-HA over expression line in Arabidopsis thaliana Col-0
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from 3-4 weeks old rosettes using the RNAeasy plant mini kit (Qiagen) and subjected to reverse transcription and amplified by in vitro transcription with t7 polymerase (Ambion).
|
Label |
Cy3
|
Label protocol |
Amplified RNA products were subjected to reverse transcription and then labeled with Cy3 and Cy5 by indirect amino-allyl method.
|
|
|
|
Hybridization protocol |
For each biological repetition, two hybridizations with swapped dye labeling were preformed.
|
Scan protocol |
Separate images for each fluorescence were acquired using ScanArray 4000 software (Packard BioScience, Meridan, CT) at a resolution of 10 mm per pixel, adjusting the photomultiplier and laser power to achieve an optimal distribution of signals without minimal saturation.
|
Description |
RNA was extracted from plants that overexpress SAS1 and from non-transformed plants. Plants were grown for 3 weeks under long day conditions. Hybridization was performed on an array from Arabidopsis Keck Resource Lab Array (Yale University) containing 12K Arabidopsis ESTs. Data in this platform contains 6 repetitions including 2 independent biological experiments.
|
Data processing |
Data analysis was performed applying per-spot
and per-chip normalization (GeneSpring 5.1; Silicon Genetics, Redwood
City, CA).
|
|
|
Submission date |
Sep 04, 2005 |
Last update date |
Mar 10, 2008 |
Contact name |
Robert Fluhr |
E-mail(s) |
robert.fluhr@weizmann.ac.il
|
Phone |
972-8-9342175
|
Organization name |
Weizmann Institute of Science
|
Department |
Plant Sciences
|
Street address |
|
City |
Rehovot |
ZIP/Postal code |
76100 |
Country |
Israel |
|
|
Platform ID |
GPL242 |
Series (1) |
GSE3246 |
A serpin, SAS1, controls hormonal cross talk in the plant defense pathway |
|