|
| Status |
Public on Apr 11, 2024 |
| Title |
1 AS sy Adi D4 (16)_(SNOWBALLs520824F) |
| Sample type |
RNA |
| |
|
| Source name |
mesenchymal stem cells
|
| Organism |
Sus scrofa |
| Characteristics |
breed: Angeln Saddleback
tissue: fibrous synovium
cell type: Adipocyte at day 4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRI Reagent (Sigma, Taufkirchen, Germany) according to the manufacturer’s protocol. After DNaseI treatment the RNA was cleaned up using the RNeasy Kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
biotin-labeled cRNAs were prepared according to the standard Affymetrix protocol from 500 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42 C on Affymetrix Porcine Snowball Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
|
| Description |
SMSCs derived from porcine synovial membrane
|
| Data processing |
Data were analysed with Affymetrix GCOS 1.1.1 software using global scaling to a target signal of 500.
|
| |
|
| Submission date |
May 14, 2023 |
| Last update date |
Apr 11, 2024 |
| Contact name |
Siriluck Ponsuksili |
| E-mail(s) |
s.wimmers@fbn-dummerstorf.de
|
| Organization name |
FBN
|
| Street address |
Wilhelm-stahl-allee 2
|
| City |
Dummerstorf |
| ZIP/Postal code |
18196 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16569 |
| Series (1) |
| GSE232501 |
Gene expression data of the adipogenesis process of mesenchymal stem cells derived from two different types of synovial membranes of German Landrace (DL) and Angeln Saddleback (AS) pigs. |
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