|
| Status |
Public on Jun 30, 2011 |
| Title |
INS-GAS male mice with H. pylori infection treated with Tamoxifen 2 |
| Sample type |
RNA |
| |
|
| Source name |
INS-GAS mouse stomach, Helicobacter pylori, Tamoxifen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: stomach
gender: male
infectious agent: Helicobacter pylori
duration of infection: 28 weeks
hormone treatment: Tamoxifen
treatment duration: 12 weeks
transgene: INS-GAS
genetic background: FVB/N
|
| Treatment protocol |
Infected with H pylori for 28 weeks. Treated with subcutaneous pellet of 0.25mg 17beta-estradiol, 15mg Tamoxfien or 5mg placebo for 12 weeks
|
| Growth protocol |
Mice maintained under normal husbandry conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol and purified with RNeasy/DNase kit
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard One-Color Microarray-Based gene expression Analysis, low input quick amp labeling protocol (AGILENT) protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on Agilent 4X44K Mouse genome array (Part #G4122F. Design #014868)
|
| Scan protocol |
GeneChips were scanned using Agilent Microarray Scanner for one color setting for the 44K array
|
| Description |
Gene expression data from INS-GAS mouse stomachs chronically infected with H. pylori and treated with Tamoxifen
|
| Data processing |
Data were quantile normalized and read with Feature Extraction software 9.1
|
| |
|
| Submission date |
Jun 03, 2011 |
| Last update date |
Jun 30, 2011 |
| Contact name |
Alexander Sheh |
| E-mail(s) |
alexsheh@mit.edu
|
| Organization name |
MIT
|
| Department |
Biological Engineering
|
| Lab |
Fox
|
| Street address |
77 Massachusetts ave 16-828
|
| City |
Cambridge |
| State/province |
ma |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE29715 |
mRNA expression data from mice infected with H. pylori treated with estradiol, tamoxifen, or placebo |
|
