|
| Status |
Public on Nov 06, 2012 |
| Title |
Stump vs Blastema [LC] |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mid-bud regenerating blastema
|
| Organism |
Ambystoma mexicanum |
| Characteristics |
days post amputation: 17
tissue: Mid-bud regenerating blastema
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using mirVana miRNA Isolation Kit (Ambion) manufacturer's instructions
|
| Label |
Cy3,Cy5
|
| Label protocol |
Cy3 or Cy5 (Outsourced to LC Sciences)
|
| |
|
| Channel 2 |
| Source name |
Non-regenerating stump
|
| Organism |
Ambystoma mexicanum |
| Characteristics |
days post amputation: 0
tissue: Non-regenerating stump
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using mirVana miRNA Isolation Kit (Ambion) manufacturer's instructions
|
| Label |
Cy3,Cy5
|
| Label protocol |
Cy3 or Cy5 (Outsourced to LC Sciences)
|
| |
|
| |
| Hybridization protocol |
using µParaflo® Microfluidic Biochip Technology
|
| Scan protocol |
Axon GenePix 4000B Microarray Scanner
|
| Description |
Biological Replicate 1 of 3:
368bl vs. 368st
Blastema_17dpa_rep1 vs. Stump_rep1
Chip: 01_YU_081977. Sample A: 368bl - Cy3. Sample B: 368st - Cy5.
Raw: 01_YU_081977-1107-Crews-368bl+368st-425-495_Data.txt.
Biological Replicate 2 of 3:
369bl vs. 369st
Blastema_17dpa_rep2 vs. Stump_rep2
Chip: 02_YU_081978. Sample A: 369st - Cy3. Sample B: 369bl - Cy5.
Raw: 02_YU_081978-1107-Crews-369st+369bl-435-505_Data.txt.
Biological Replicate 3 of 3:
378bl vs. 378st
Blastema_17dpa_rep3 vs. Stump_rep3
Chip: 03_YU_081979. Sample A: 378st - Cy3. Sample B: 378bl - Cy5.
Raw: 03_YU_081979-1107-Crews-378st+378bl-450-485_Data.txt.
|
| Data processing |
ArrayPro software-Global Normalization
|
| |
|
| Submission date |
Jun 03, 2011 |
| Last update date |
Nov 06, 2012 |
| Contact name |
Edna C Holman |
| E-mail(s) |
edna96@hotmail.com
|
| Phone |
203-432-6553
|
| URL |
http://www.yale.edu/crews/
|
| Organization name |
Yale University
|
| Department |
Molecular, Cellular and Developmental Biology
|
| Lab |
Craig Crews
|
| Street address |
266 Whitney Ave. KBT-442
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platform ID |
GPL13686 |
| Series (1) |
| GSE29727 |
Microarray Analysis of microRNA Expression during Axolotl Limb Regeneration |
|
