|
| Status |
Public on Nov 28, 2011 |
| Title |
pathogen isolates: Mock - time: 12 hai - rep1 |
| Sample type |
RNA |
| |
|
| Source name |
leaf (pathogen isolates: Mock - time: 12 hai)
|
| Organism |
Glycine max |
| Characteristics |
pathogen isolates: Mock
time: 12 hr
germplasm name: pi462312
organism part: po:0009025 leaf
development stage: PO:0007134 A vegetative growth
growth media: soil mix
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol-Qiagen
|
| Label |
biotin
|
| Label protocol |
Affy-Eukaryotic Target Preparation
|
| |
|
| Hybridization protocol |
Affy- Eukaryotic Target Hybridization
|
| Scan protocol |
Affy- Array Scanning
|
| Description |
PI462312 mock treatment 12 hours
|
| Data processing |
The MAS5.0 Normalization is performed using the justMAS function in the simpleaffy package of Bioconductor (www.bioconductor.org). The target value is 500 for all cases. The code was developed using R2.4.1, Bioconductor 1.9, and simpleaffy 2.8.0. The CDF files were downloaded from the Bioconductor website at http://www.bioconductor.org/packages/release/AffymetrixChip.html. The normalization scripts are available from the PLEXdb team by request.
|
| |
|
| Submission date |
Jun 03, 2011 |
| Last update date |
Nov 28, 2011 |
| Contact name |
PLEXdb Curator, Sudhansu Dash |
| Organization name |
Iowa State University
|
| Department |
Virtual Reality Application Center
|
| Lab |
PLEXdb
|
| Street address |
1023 Crop Genomes Informatics Lab (Iowa State University)
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
|
| Platform ID |
GPL4592 |
| Series (1) |
| GSE29740 |
Molecular characterization of the incompatible and compatible interaction of soybean rust disease in Rpp3-containing PI462312 line |
|
