|
| Status |
Public on May 16, 2013 |
| Title |
CTX_A2B5+_03 |
| Sample type |
RNA |
| |
|
| Source name |
cortex, glial progenitor cell, A2B5+ sort
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: cortex
pathology: epilepsy
sort population: A2B5+
cell type: glial progenitor cell
tumor grade (who): non-tumor
|
| Treatment protocol |
Glioma and non-neoplastic A2B5+ GPCs were isolated using magnetic-beads (MACS) or fluorescent-activated (FACS) cell sorting followed by confirmation of the purity of the separated cell populations. CD11b+ microglia were isolated from adult human non-neoplastic tissue using a similar procedure. . The purity of sorted CD11b+ microglia was verified using RCA-lectin staining (Vector Labs). Acutely isolated cells were frozen for RNA extraction
|
| Growth protocol |
Adult human white matter and cortex tissues from epileptic patients and glioma tissue were dissociated using papain. Glial progenitor cells derived from adult human brain and glioma were cultured in DMEM/F12/N2 media supplemented with bFGF, EGF, and PDGF-AA (20ng/ml) in cell suspension plates.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Nugen Ovation biotin labeling system using the standard protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol for U133 Plus 2.0 Arrays
|
| Scan protocol |
Standard Affymetrix protocol for U133 Plus 2.0 Arrays
|
| Description |
FJS319_Goldman_U133Plus_2
normal
|
| Data processing |
Affymetrix .cel raw data files were normalized using RMA. All data processing was performed using R and the Bioconductor package.
|
| |
|
| Submission date |
Jun 07, 2011 |
| Last update date |
May 16, 2013 |
| Contact name |
Steven Goldman |
| E-mail(s) |
Steven_Goldman@urmc.rochester.edu
|
| Organization name |
University of Rochester
|
| Department |
Center for Translational Neuromedicine
|
| Lab |
Goldman Lab
|
| Street address |
601 Elmwood Ave
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE29796 |
Transcriptional Differences between Normal and Glioma-Derived Glial Progenitor Cells Identify a Core Set of Dysregulated Genes. |
|
