ecotype: Col-0 genotype: hyl1-2 tissue: flowers and flower buds
Growth protocol
12hr light / 12hr dark, 21C.
Extracted molecule
total RNA
Extraction protocol
Life Tech Total RNA-seq kit, total RNA modification, per manufacturer's instructions.
Library strategy
RNA-Seq
Library source
transcriptomic
Library selection
size fractionation
Instrument model
AB SOLiD 4 System
Description
hyl_flower
Data processing
The raw colorspace reads containing inserts derived from 20-25nt RNAs were identified by searching for 3' matches (allowing up to two mismatches) to the sequencing adapter sequence (in colorspace); the inferred adapter sequences were then trimmed, leaving 20-25nt colorspace reads. These data were then translated into DNA space while simultaneously discarding any reads with ambiguous (e.g., one or more "." characters) data. The data in the Sample data tables are simple counts of each of these 20-25nt total RNAs.
