|
Status |
Public on Aug 31, 2011 |
Title |
534-11-131 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
TN+ ADC
|
Organism |
Homo sapiens |
Characteristics |
gender: male age: 63y smoker: yes Stage: 1
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
Label |
CY3
|
Label protocol |
10ug amplified RNA(aRNA) from tumor cells was labeled indirectly with CY3 and the same amout of human reference RNA labeled with CY5
|
|
|
Channel 2 |
Source name |
Universal reference RNA (URR)
|
Organism |
Homo sapiens |
Characteristics |
sample type: pool of 10 human carcinoma cell lines, including colon, breast, brain, lung ovarian carcinoma cells as a controlling intra- and inter-experimental variation from Stratagene.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
Label |
CY5
|
Label protocol |
10ug amplified RNA(aRNA) from tumor cells was labeled indirectly with CY3 and the same amout of human reference RNA labeled with CY5
|
|
|
|
Hybridization protocol |
Dual hybridisation of tumor sample labeled with Cy3 in ch 1 and URR labeled with Cy5 in ch 2 onto cDNA microarray slide. After hybridization, slides were washed sequential.
|
Scan protocol |
Scanned on a GSI Lumonics 4000 Scanner
|
Description |
laser captue microdissected tumor epithelial cells RNA for ch1 and URR for ch2 co-hybridisation
|
Data processing |
Images were quantified using and ScanArray software (PerkinElmer Life Science Inc., Boston, Massachusetts). GeneSpring 6.1(Agilent) software was used.
|
|
|
Submission date |
Jun 08, 2011 |
Last update date |
Aug 31, 2011 |
Contact name |
Jiangting Hu |
E-mail(s) |
jianting.hu@ndcls.ox.ac.uk
|
Phone |
+44 1865 221763
|
Organization name |
University of Oxford
|
Department |
NDCLS
|
Lab |
CRUK Tumour Pathology
|
Street address |
Headley Way
|
City |
Oxford |
State/province |
Oxfordshire |
ZIP/Postal code |
OX3 9DU |
Country |
United Kingdom |
|
|
Platform ID |
GPL334 |
Series (1) |
GSE29827 |
Human lung ADC and SCC comparsion study |
|