|
| Status |
Public on Jul 20, 2011 |
| Title |
Gcn4Δ mutant cells |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
BY4741 Gcn4Δ - untreated
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BY4741
genotype/variation: Gcn4Δ
|
| Treatment protocol |
20 mM boric acid treatment for 1 hour
|
| Growth protocol |
BY4741 wild type, yap1Δ, gcn2Δ, gcn4Δ mutant cells were grown to an optical density of 0.2-0.3 at 600 nm in 200 ml YPD medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion Yeast RNA purification kit
|
| Label |
CY3
|
| Label protocol |
Agilent standard labeling protocol
|
| |
|
| Channel 2 |
| Source name |
BY4741 Gcn4Δ + 20mM boric acid
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BY4741
genotype/variation: Gcn4Δ
|
| Treatment protocol |
20 mM boric acid treatment for 1 hour
|
| Growth protocol |
BY4741 wild type, yap1Δ, gcn2Δ, gcn4Δ mutant cells were grown to an optical density of 0.2-0.3 at 600 nm in 200 ml YPD medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion Yeast RNA purification kit
|
| Label |
CY5
|
| Label protocol |
Agilent standard labeling protocol
|
| |
|
| |
| Hybridization protocol |
Agilent standard hybridization protocol
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jun 10, 2011 |
| Last update date |
Jul 20, 2011 |
| Contact name |
Dmitri E Fomenko |
| E-mail(s) |
dfomenko@genomics.unl.edu
|
| Organization name |
University of Nebraska
|
| Department |
Biochemistry
|
| Street address |
1901 Vine, N138
|
| City |
Lincoln |
| State/province |
NE |
| ZIP/Postal code |
68588 |
| Country |
USA |
| |
|
| Platform ID |
GPL7542 |
| Series (1) |
| GSE29887 |
ATR1 Boron Transporter is Regulated by Gcn4 Transcription Factor in Response to Boron Stress |
|
