|
| Status |
Public on Jun 15, 2011 |
| Title |
T1525 cell line |
| Sample type |
RNA |
| |
|
| Source name |
Prostate tumor from TRAMP mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
genotype/variation: TRAMP
age: 30 wks
tissue origin: Prostate
tissue type: tumor cell line
cell line: T1525
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the guanidinium thiocyanate/cesium chloride gradient method, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina RNA TotalPrep amplification kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalised using quantile normalisation with Illumina BeadStudio Gene Expression Module v3
|
| |
|
| Submission date |
Jun 14, 2011 |
| Last update date |
Jun 15, 2011 |
| Contact name |
Paola Pittoni |
| E-mail(s) |
paola.pittoni@istitutotumori.mi.it
|
| Phone |
+39 0223903232
|
| Fax |
+39 0223902630
|
| Organization name |
IRCCS Istituto Nazionale dei Tumori
|
| Department |
Dept. Experimental Oncology
|
| Lab |
Molecular Immunology Unit
|
| Street address |
Via Amadeo, 42
|
| City |
Milan |
| State/province |
Italy |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6103 |
| Series (2) |
| GSE29957 |
Gene expression analysis of 2 different prostate tumor cell lines isolated from 30 wks old TRAMP mice compared to normal prostate |
| GSE29958 |
Mast cell targeting hampers prostate adenocarcinoma development but promotes the occurrence of highly malignant neuroendocrine cancers. |
|
