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| Status |
Public on Jan 08, 2024 |
| Title |
6h_2 |
| Sample type |
SRA |
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| Source name |
H99
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| Organism |
Cryptococcus neoformans var. grubii H99 |
| Characteristics |
strain: H99
genotype: WT
treatment: YPD liquid medium, 6h
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
The level and integrity of RNA in each sample were evaluated using a Qubit RNA Assay Kit on a Qubit 2.0 Flurometer (Life Technologies, CA, USA) and RNA Nano 6000 Assay Kit with the Bioanalyzer 2100 system (Agilent Technologies, CA, USA), respectively. RNA purity was assessed using a Nano Photometer spectrophotometer (IMPLEN, CA, USA).
The transcriptome libraries were generated using the VAHTS mRNA-seq v2 Library Prep Kit (Vazyme Biotech Co., Ltd, Nanjing, China) according to the manufacturer’s instructions.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
FastQC v0.11.5
trimmomatic v0.39
Hisat2 v2.1.0
Stringtie v1.3.3
DEseq2 v1.16.1
Assembly: Cryptococcus neoformans var. grubii H99
Supplementary files format and content: tpm TPM of all strains
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| Submission date |
May 27, 2023 |
| Last update date |
Jan 08, 2024 |
| Contact name |
hao ding |
| E-mail(s) |
dinghaov587@126.com
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| Organization name |
institute of microbiology
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| Street address |
BeiChen Road
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platform ID |
GPL33437 |
| Series (1) |
| GSE233619 |
Fungicide-tolerant persister formation during cryptococcal pulmonary infection |
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| Relations |
| BioSample |
SAMN35444807 |
| SRA |
SRX20528746 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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