|
| Status |
Public on May 01, 2024 |
| Title |
KO_48_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Gut and salivary gland
|
| Organism |
Ixodes scapularis |
| Characteristics |
tissue: Gut and salivary gland
treatment: Feeding on adiponectin knockout mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tick gut and salivary gland was dissected, and RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Tick transcript data was downloaded from the VectorBase (https://vectorbase.org/vectorbase/app/) and indexed using the kallisto-index.
The reads from the sequencer were pseudo-aligned with the index reference transcriptome using kallisto.
The counts generated were processed by DESeq2 in RStudio (https://rstudio.com).
Assembly: IscaW1
Supplementary files format and content: tab-delimited text file includes tpm value for each gene
|
| |
|
| Submission date |
May 29, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
Xiaotian Tang |
| E-mail(s) |
tangxt@zju.edu.cn
|
| Organization name |
Zhejiang University
|
| Department |
Entomology
|
| Street address |
Agro-Bio-Environ Building, Zhejiang University
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310030 |
| Country |
China |
| |
|
| Platform ID |
GPL29893 |
| Series (1) |
| GSE233660 |
Adiponectin inhibits acquisition of the Lyme disease agent by ticks [Tick] |
|
| Relations |
| BioSample |
SAMN35522636 |
| SRA |
SRX20533877 |
