|
Status |
Public on May 20, 2024 |
Title |
WK-521_HiLung-Airway_1 |
Sample type |
SRA |
|
|
Source name |
iPSC-derived airway cell
|
Organism |
Homo sapiens |
Characteristics |
tissue: iPSC-derived airway cell
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using QIAamp RNA Blood Mini Kit (Qiagen, Cat# 52304) RNA libraries for RNA-seq were prepared using TruSeq Stranded mRNA Library Prep Kit (illumina) following manufacturer's protocols.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Sequenced reads were trimmed with fastp (v.0.21.0). Sequenced reads were mapped to the human, SARS-CoV-2, and BANAL-236 reference genomes using STAR (v.2.6.1c). Assembly: Human (GRCh38.p13), BANAL-236 (MZ937003.2), and SARS-CoV-2 (NC_045512.2) Supplementary files format and content: Expression read count matrix (counts.txt)
|
|
|
Submission date |
Jun 01, 2023 |
Last update date |
May 20, 2024 |
Contact name |
Kei Sato |
E-mail(s) |
su9ark@gmail.com
|
Phone |
81364092212
|
Organization name |
The Institute of Medical Science, The University of Tokyo
|
Department |
Department of Microbiology and Immunology
|
Lab |
Division of Systems Virology
|
Street address |
4-6-1 Shiroganedai
|
City |
Minato-ku |
State/province |
Tokyo |
ZIP/Postal code |
1088639 |
Country |
Japan |
|
|
Platform ID |
GPL24676 |
Series (1) |
GSE233943 |
RNA-Seq data from iPSC-derived human airway cells and colon organoids infected with BANAL-20-236, WK-521, and WK-521 dFCS |
|
Relations |
BioSample |
SAMN35561237 |
SRA |
SRX20571445 |