|
| Status |
Public on Dec 31, 2012 |
| Title |
p80HT transgenic 1 |
| Sample type |
RNA |
| |
|
| Source name |
p80HT Mutation
|
| Organism |
Mus musculus |
| Characteristics |
genotype: p80HT transgenic
genetic background: C57BL/6J x SJL/J
cell type: B cell
|
| Treatment protocol |
B cells were isolated from the spleens of mice with EasySep (Stemcell Technologies, Vancouver, BC, Canada) using an anti B220 antibody (clone RA3-6B2, BD Pharmingen, San Diego, CA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction by manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Samples were labeled using the WT Terminal Labeling kit (Affymetrix, Santa Clara, CA) with using terminal deoxynucleotidyl transferase (TdT) in the presence of a biotinylated compound
|
| |
|
| Hybridization protocol |
Hyb mix included Control Oligonucleotide B2, 20X Eukaryotic Hyb Controls and DMSO (Affymetrix, Santa Clara, CA). 200ul of mixed sample was hybridized for 18 hours at 45C. The arrays are then washed and stained with streptavidin phycoerythrin conjugate using an automated protocol on the GeneChip® Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned using Affymetrix GeneChip Scanner 3000. Images were aquired with AGCC software.
|
| Description |
p80HT Mutation
|
| Data processing |
The arrays were nomalized (RMA) using Partek. The sample groups were compared using ANOVA, from which genes with a 2-fold-change and significant p-values were isolated.
|
| |
|
| Submission date |
Jun 18, 2011 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Brian A McCarthy |
| E-mail(s) |
bmcc333@gmail.com
|
| Phone |
347-256-3876
|
| Organization name |
Georgia Health Sciences University
|
| Department |
Cancer Center
|
| Lab |
HF Ding
|
| Street address |
1410 Laney Walker Blvd.
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE30080 |
NF-kB2 mutation targets survival, proliferation and differentiation pathways in the pathogenesis of B-lineage lymphomas |
|
