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Sample GSM74512 Query DataSets for GSM74512
Status Public on Sep 21, 2005
Title Heat-shock(Temp43, 30min)-3, (high power scan)
Sample type RNA
 
Channel 1
Source name Heat-shock(Temp43, 30min), Cy5
Organism Saccharomyces cerevisiae
Characteristics strain JN54 (wild-type)
Biomaterial provider Craig, EA
Growth protocol JN54 (wild-type) cells were incubated in YPD medium at 30 degrees C to a logarithmic phase (OD660=1), followed by treatment with mild heat-shock at 43 degrees C for 30 min in pre-warmed (43 degrees C) 100ml of YPD medium using 500 ml Erlenmeyer flask.
Extracted molecule polyA RNA
Extraction protocol Hot-phenol and oligotex dt mRNA purification kit (TaKaRa)
Label Cy5
Label protocol Cyscribe first-strand, cDNA labeling kit (Amersham Biosciences)
 
Channel 2
Source name Control (temp30, 30min), Cy3
Organism Saccharomyces cerevisiae
Characteristics strain JN54 (wild-type)
Biomaterial provider Craig, EA
Growth protocol JN54 (wild-type) cells were incubated in YPD medium at 30 degrees C to a logarithmic phase (OD660=1), followed by incubated at 30 degrees C for 30 min in 100ml of YPD medium using 500 ml Erlenmeyer flask.
Extracted molecule polyA RNA
Extraction protocol Hot-phenol and oligotex dt mRNA purification kit (TaKaRa)
Label Cy3
Label protocol Cyscribe first-strand, cDNA labeling kit (Amersham Biosciences)
 
 
Hybridization protocol Synthesized cDNA were hybridized to Kuhara DNA chip (DNA Chip Research, Yokohama, Kanagawa, Japan) at 65 degrees C for 48 h.
Description The ratio of intensity Cy5/Cy3 was calculated and normalized with negative control spots. In order to rescue data that had a higher intensity than the scanning range, the array were scanned twice with different laser power. This data is result from high power scanning.
Data processing GeneSpring (Silicon Genetics)
 
Submission date Sep 16, 2005
Last update date Apr 28, 2006
Contact name Rena Matsumoto
E-mail(s) matsumoto-rn@aist.go.jp
Phone +81 29 861 6075
Fax +81 29 861 6689
Organization name National Institute of Advanced Industrial Science and Technology
Street address 1-1-1 Higashi
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8566
Country Japan
 
Platform ID GPL1945
Series (1)
GSE3316 Heat-shock (Temp43, 30min, 60 min)

Data table header descriptions
ID_REF
VALUE Normalized log ratio (log2)
Cy5F Cy5 inner intensity (median)
Cy5B Cy5 intensity surrounding each spot (background), (median)
Cy3F Cy3 inner intensity (median)
Cy3B Cy3 intensity surrounding each spot (background), (median)

Data table
ID_REF VALUE Cy5F Cy5B Cy3F Cy3B
1010101 -0.598504381 85 34 100 32
1010102 -0.050200351 68 34 63 32
1010103 -1.431394395 58 34 89 32
1010104 -0.320970405 54 34 54 32
1010105 -0.183466882 53 34 52 33
1010106 -0.431394395 83 35 90 33
1010201 0.002946243 67 34 62 33
1010202 -0.141646706 69 34 67 33
1010203 -0.335469975 43 34 43 33
1010204 null 52 35 57 33
1010205 -0.343931554 51 34 52 33
1010206 -0.546036961 56 35 60 33
1010301 -0.076551678 91 35 85 33
1010302 -0.183466882 70 34 68 32
1010303 -0.335469975 52 34 53 33
1010304 -0.389917759 47 34 48 33
1010305 -0.483027163 48 35 49 33
1010306 -0.394970987 54 35 55 33
1010401 -0.598504381 41 35 41 33
1010402 -0.122066337 83 35 79 33

Total number of rows: 6912

Table truncated, full table size 222 Kbytes.




Supplementary data files not provided

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