|
| Status |
Public on Sep 21, 2005 |
| Title |
Heat-shock(Temp43, 60min)-3, (low power scan) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Heat-shock(Temp43, 60min), Cy5
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain JN54 (wild-type)
|
| Biomaterial provider |
Craig, EA
|
| Growth protocol |
JN54 (wild-type) cells were incubated in YPD medium at 30 degrees C to a logarithmic phase (OD660=1), followed by treatment with mild heat-shock at 43 degrees C for 60 min in pre-warmed (43 degrees C) 100ml of YPD medium using 500 ml Erlenmeyer flask.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Hot-phenol and oligotex dt mRNA purification kit (TaKaRa)
|
| Label |
Cy5
|
| Label protocol |
Cyscribe first-strand, cDNA labeling kit (Amersham Biosciences)
|
| |
|
| Channel 2 |
| Source name |
Control (temp30, 60min), Cy3
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain JN54 (wild-type)
|
| Biomaterial provider |
Craig, EA
|
| Growth protocol |
JN54 (wild-type) cells were incubated in YPD medium at 30 degrees C to a logarithmic phase (OD660=1), followed by incubated at 30 degrees C for 60 min in 100ml of YPD medium using 500 ml Erlenmeyer flask.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Hot-phenol and oligotex dt mRNA purification kit (TaKaRa)
|
| Label |
Cy3
|
| Label protocol |
Cyscribe first-strand, cDNA labeling kit (Amersham Biosciences)
|
| |
|
| |
| Hybridization protocol |
Synthesized cDNA were hybridized to Kuhara DNA chip (DNA Chip Research, Yokohama, Kanagawa, Japan) at 65 degrees C for 48 h.
|
| Description |
The ratio of intensity Cy5/Cy3 was calculated and normalized with negative control spots. In order to rescue data that had a higher intensity than the scanning range, the array were scanned twice with different laser power. This data is result from low power scanning.
|
| Data processing |
GeneSpring (Silicon Genetics)
|
| |
|
| Submission date |
Sep 16, 2005 |
| Last update date |
Apr 28, 2006 |
| Contact name |
Rena Matsumoto |
| E-mail(s) |
matsumoto-rn@aist.go.jp
|
| Phone |
+81 29 861 6075
|
| Fax |
+81 29 861 6689
|
| Organization name |
National Institute of Advanced Industrial Science and Technology
|
| Street address |
1-1-1 Higashi
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1945 |
| Series (1) |
| GSE3316 |
Heat-shock (Temp43, 30min, 60 min) |
|
