|
| Status |
Public on Jan 18, 2012 |
| Title |
Adipose TZD rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Adipose progenitors, TZD, 2month
|
| Organism |
Mus musculus |
| Characteristics |
source tissue: white adipose tissue
developmental stage: adult (2-6 month old)
cell type: GFP+ stromal-vascular cells
strain: PPARgamma-tTA;tetO-H2B-GFP
treatment: rosiglitazone
|
| Treatment protocol |
Mice were fed with normal chow or rosiglitazone-containing normal chow (0.0075%) for 2 months.
|
| Growth protocol |
Adult (2-6 months old) PPARgamma-tTA;tetO-H2B-GFP mice were housed in a 12:12 light:dark cycle and chow and water were provided ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
White adipose tissues (inguinal, interscapular and gonadal white adipose tissues) were digested with collagenase for 2 hours at 37 degree with gentle shaking, and stromal-vascular fraction was collected and GFP+ cells from this fraction were FACS-sorted. RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI
|
| |
|
| Submission date |
Jun 21, 2011 |
| Last update date |
Jan 18, 2012 |
| Contact name |
Wei Tang |
| E-mail(s) |
wei.tang@utsouthwestern.edu
|
| Phone |
214-648-1482
|
| Fax |
214-648-1960
|
| Organization name |
UT Southwestern Medical Center at Dallas
|
| Department |
Developmental Biology
|
| Lab |
Jonathan M. Graff
|
| Street address |
6000 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE30116 |
Rosiglitazone-induced molecular changes in adipose progenitors |
|
