|
| Status |
Public on Jun 09, 2023 |
| Title |
39C2-2 |
| Sample type |
SRA |
| |
|
| Source name |
CAR-2CCT-T
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: 2rounds of stimulation
cell type: CAR-2CCT-T
|
| Extracted molecule |
total RNA |
| Extraction protocol |
positive CAR-T cell total RNA was extracted using RNeasy Plus mini isolation kit (Qiagen).
Library preparations were performed using a NEBNext® Ultra™RNA Library Prep Kit for Illumina according to manufacturer’s instruction.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Biol rep2
|
| Data processing |
Fastq files generated by sequencing were aligned and mapped using STAR with the human genome assembly version GRCh38.
Assembly: Ensembl GRCh38
Supplementary files format and content: Tab-delimited text files contain read counts after alignment.
|
| |
|
| Submission date |
Jun 06, 2023 |
| Last update date |
Jun 09, 2023 |
| Contact name |
Sidi Chen |
| E-mail(s) |
sidi.chen@yale.edu
|
| Organization name |
Yale University
|
| Street address |
850 West Campus Drive
|
| City |
West Haven |
| State/province |
CT |
| ZIP/Postal code |
06516 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE234306 |
CTLA-4 tail fusion enhances CAR-T anti-tumor immunity [bulk RNA-seq] |
| GSE234307 |
CTLA-4 tail fusion enhances CAR-T anti-tumor immunity |
|
| Relations |
| BioSample |
SAMN35652119 |
| SRA |
SRX20607765 |
