|
Status |
Public on Sep 06, 2023 |
Title |
endometrial biopsy, X210347 |
Sample type |
RNA |
|
|
Source name |
Human endometrium
|
Organism |
Homo sapiens |
Characteristics |
tissue: Human endometrium age: 28 cycle stage: 6 pod: NA batch: batch 5
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from homogenized endometrial tissues using RNA lysis solution (RLT buffer) and RNeasy Plus Mini Kit according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared using the Ambion Illumina TotalPrep RNA amplification kit.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol with Illumina iScan Reader.
|
Description |
Sample X210347 from batch 5
|
Data processing |
Data generated by Illumina iScan Reader was processed by Illumina GenomeStudio software. The data was then normalized in R using background normalization and robust spline normalization with the lumi package and batch corrected with ComBat using the sva package.
|
|
|
Submission date |
Jun 07, 2023 |
Last update date |
Sep 06, 2023 |
Contact name |
Peter Rogers |
Organization name |
University of Melbourne
|
Department |
Department of Obstetrics and Gynaecology
|
Street address |
Level 7, University Department, Royal Women's Hospital, Crn Grattan Street and Flemington Rd
|
City |
Parkville |
State/province |
VIC |
ZIP/Postal code |
3052 |
Country |
Australia |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE234368 |
Gene expression profiles of endometrium from women across the menstrual cycle |
GSE234434 |
A molecular staging model for accurately dating the endometrial biopsy |
|