|
| Status |
Public on May 24, 2013 |
| Title |
hinny3702_day33_CG_RNAseq |
| Sample type |
SRA |
| |
|
| Source name |
hinny embryonic day 33 chorionic girdle sample
|
| Organism |
Equus caballus x Equus asinus |
| Characteristics |
tissue: chorionic girdle
developmental stage: embryonic day 33
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The mRNA-seq libraries were made with 2.5 ug of starting total RNA samples using the mRNA-Seq 8-Sample Prep Kit (Illumina Inc., CA), following the Illumina protocol for mRNA sequencing sample preparation.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Hinny: Offspring of male horse and female donkey.
Illumina mRNA-seq
|
| Data processing |
Alignment: The reads were truncated to 40 bp and aligned to the horse reference genome (UCSC version equCab2) using Burrows-Wheeler Alignment (BWA) with a maximum of four mismatches. On average, 67.1% of the reads were mapped to the horse reference genome.
|
| |
|
| Submission date |
Jun 27, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrew Clark |
| E-mail(s) |
ac347@cornell.edu
|
| Organization name |
Cornell University
|
| Street address |
227 Biotech Building
|
| City |
Ithaca |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
|
| Platform ID |
GPL13777 |
| Series (1) |
| GSE30243 |
Profiling of differential allelic expression in horse, donkey, mule and hinny placental tissue |
|
| Relations |
| SRA |
SRX080110 |
| BioSample |
SAMN00631159 |
