|
Status |
Public on Jul 06, 2023 |
Title |
FR_RNA_T1D_HU_20191219_P2_CD4T-cells-96-single_cell_sorted_cells |
Sample type |
RNA |
|
|
Source name |
Blood
|
Organism |
Homo sapiens |
Characteristics |
dq8_tetramer: _12-20_, _13-21_, _CP11_ or_CP18_
|
Extracted molecule |
total RNA |
Extraction protocol |
Peripheral Blood Mononuclear Cells (PBMCs) were isolated via Ficoll gradient from blood isolated from all donors
|
Label |
EVAGREEN
|
Label protocol |
Per donor: PBMCs were split and stained with either tetramer for 1 hour at 4C in the dark. Washed twice with FACS Wash (2% FCS, 2 mM EDTA, 1x PBS) and then stained with surface antibodies for 30 minutes at 4C in the dark.
|
|
|
Hybridization protocol |
n/a
|
Scan protocol |
n/a
|
Description |
CD4+ T cell
|
Data processing |
Data was processed using custom R scripts
|
|
|
Submission date |
Jun 16, 2023 |
Last update date |
Jul 06, 2023 |
Contact name |
Luc Teyton |
E-mail(s) |
lteyton@scripps.edu
|
Organization name |
Scripps Research
|
Department |
Immunology and Microbiology
|
Lab |
Teyton Lab
|
Street address |
10550 North Torrey Pines Road
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL33499 |
Series (2) |
GSE235123 |
Measuring anti-islet autoimmunity in mouse and human by profiling peripheral blood antigen specific CD4 T cells [Fluidigm] |
GSE235667 |
Measuring anti-islet autoimmunity in mouse and human by profiling peripheral blood antigen specific CD4 T cells |
|