|
| Status |
Public on Jul 06, 2023 |
| Title |
FR_RNA_T1D_HU_20200303_P2_CD4T-cells-96-single_cell_sorted_cells |
| Sample type |
RNA |
| |
|
| Source name |
Blood
|
| Organism |
Homo sapiens |
| Characteristics |
dq8_tetramer: _12-20_, _13-21_, _CP11_ or_CP18_
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Peripheral Blood Mononuclear Cells (PBMCs) were isolated via Ficoll gradient from blood isolated from all donors
|
| Label |
EVAGREEN
|
| Label protocol |
Per donor: PBMCs were split and stained with either tetramer for 1 hour at 4C in the dark. Washed twice with FACS Wash (2% FCS, 2 mM EDTA, 1x PBS) and then stained with surface antibodies for 30 minutes at 4C in the dark.
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
CD4+ T cell
|
| Data processing |
Data was processed using custom R scripts
|
| |
|
| Submission date |
Jun 16, 2023 |
| Last update date |
Jul 06, 2023 |
| Contact name |
Luc Teyton |
| E-mail(s) |
lteyton@scripps.edu
|
| Organization name |
Scripps Research
|
| Department |
Immunology and Microbiology
|
| Lab |
Teyton Lab
|
| Street address |
10550 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL33499 |
| Series (2) |
| GSE235123 |
Measuring anti-islet autoimmunity in mouse and human by profiling peripheral blood antigen specific CD4 T cells [Fluidigm] |
| GSE235667 |
Measuring anti-islet autoimmunity in mouse and human by profiling peripheral blood antigen specific CD4 T cells |
|
