|
| Status |
Public on Jul 12, 2023 |
| Title |
Control5_M_T |
| Sample type |
SRA |
| |
|
| Source name |
lung
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lung
cell type: total lung
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared with Trizol reagent according to manufacturer's instructions.
low-input mRNA-Seq; Libraries were generated using NEBNext® NEBNext Single Cell/Low Input RNA Library Prep Kit according to manufacturer's instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
paired-end run was performed using a NextSeqHigh
Most steps of this pipeline were performed using the Galaxy platform.
Reads were trimmed with Flexbar 3.5.0. and aligned to human reference genome (hg38) using HISAT 2.1.0. and for transcript assembly and quantification StringTie 2.1.1 was used.
Assembly: hg38
Supplementary files format and content: raw gene counts in txt.format
|
| |
|
| Submission date |
Jun 16, 2023 |
| Last update date |
Jul 12, 2023 |
| Contact name |
Stefan Engelhardt |
| Organization name |
Technical University Munich
|
| Department |
Institute of Pharmacology and Toxicology
|
| Street address |
Biedersteiner Str. 29
|
| City |
Munich |
| ZIP/Postal code |
80802 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE235133 |
Trimannose-coupled antimiR-21 for macrophage-targeted inhalation treatment of acute inflammatory lung damage [transcriptome_human_COVID] |
| GSE235136 |
Trimannose-coupled antimiR-21 for macrophage-targeted inhalation treatment of acute inflammatory lung damage |
|
| Relations |
| BioSample |
SAMN35774971 |
| SRA |
SRX20709141 |
