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Status |
Public on Jun 30, 2011 |
Title |
sh-JMJD1B_rep2 |
Sample type |
RNA |
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|
Source name |
sh-JMJD1B
|
Organism |
Homo sapiens |
Characteristics |
cell line: K562 genotype/variation: JMJD1B knock-down
|
Treatment protocol |
Stable cells were treated 1 ug/ml doxyclcline for 48 h.
|
Growth protocol |
K562 cells in RPMI1640 medium supplemented with 5 % fetal bovine serum (FBS) and 0.05 % antibiotics at 37 C in a 5 % CO2 atmosphere
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared from 0.55 ug total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin,TX).
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Hybridization protocol |
Following fragmentation, 0.75 ug of cRNA were hybridized to the Illumina HumanHT-12 Expression Beadchip according to the protocols provided by the manufacturer.
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Scan protocol |
Arrays were scanned using the Illumina Bead Array ReaderĀ Confocal Scanner
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Description |
Tet-on system
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Data processing |
Array data export processing and analysis was performed using Illumina GenomeStudio v2009.2 Gene Expression Module v1.5.4.Array data were filtered by detection p-value < 0.05 (similar to signal to noise) and the selected probe signal values were transformed by logarithm and normalized by quantile method.
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Submission date |
Jun 29, 2011 |
Last update date |
May 17, 2012 |
Contact name |
jiyoung kim |
E-mail(s) |
gene5486@hanmail.net
|
Organization name |
Chung-Ang University
|
Street address |
Heusokdong
|
City |
seoul |
ZIP/Postal code |
156-756 |
Country |
South Korea |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE30294 |
JMJD1B target gene screening :JMJD1B is the H3K9 demethylase involved in transcriptional activation of Lmo2 in leukemia |
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