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Sample GSM752 Query DataSets for GSM752
Status Public on Nov 28, 2001
Title SAGE_SciencePark_MCF7_estradiol_10h
Sample type SAGE
Anchor NlaIII
Tag Count 60725
Tag Length 10
Source name Breast Carcinoma - Pleaural effusion
Organism Homo sapiens
Extracted molecule total RNA
Description MCF7 cells were grown in conditions devoid of estrogen for 48h - (IMEM media supplemented w/ 10% Charcoal Stripped Serum) at time 0h cells were treated with 10-8 M estradiol (E2) - This SAGE library represents the 10h time-point of E2 treatment
Sex: Female
Organ: Breast
Cell_type: epithelial
Cell_line: MCF7
Lab host: Top10 bacteria
Vector: pZero
Vector type: plasmid
R. Site 1: NlaIII
R. Site 2: BsmF1 as tagging enzyme
Sequences generated to date: 2324

This library represents a Cancer Genome Anatomy Project library, which was either produced through CGAP funding, or donated to CGAP.
The Cancer Genome Anatomy Project (CGAP: is an interdisciplinary program established and administered by the National Cancer Institute (NCI: to generate the information and technological tools needed to decipher the molecular anatomy of the cancer cell.
Keywords = mammary gland
Keywords = carcinoma
Keywords = cell line
Keywords = estrogen
Keywords = EST
Submission date Nov 28, 2001
Last update date May 28, 2005
Contact name C Marcelo Aldaz
Phone 512-237-9530
Organization name M.D. Anderson Cancer Center
Department Science Park Research Division, Carcinogenesis
Street address
City Smithville
State/province TX
ZIP/Postal code 78957
Country USA
Platform ID GPL4
Series (1)
GSE41 Effects of estrogen

Data table header descriptions
TAG Ten base SAGE tag,
COUNT Absolute tag count
TPM Tags per million, or (1000000*COUNT)/(Total tags)

Data table
CCTCCAGCTA 760 12515.4
TTGGTCCTCT 625 10292.3
CCAGAACAGA 584 9617.13
GCATAATAGG 377 6208.32
CTGGGTTAAT 355 5846.03
CAAATGAGGA 352 5796.62
AAGACAGTGG 339 5582.54
CACAAACGGT 339 5582.54
GTGAAGGCAG 324 5335.53
ACTTTTTCAA 323 5319.06
AAGGAGATGG 292 4808.56
GCCTGTATGA 291 4792.1
AAGGTGGAGG 287 4726.22
GTGCTGAATG 276 4545.08
ATAATTCTTT 273 4495.68
TGGTGTTGAG 267 4396.87
TACCATCAAT 266 4380.4
AGGCTACGGA 258 4248.66
ACATCATCGA 257 4232.19
TGTGTTGAGA 250 4116.92

Total number of rows: 17213

Table truncated, full table size 352 Kbytes.

Supplementary data files not provided

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