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Sample GSM75265 Query DataSets for GSM75265
Status Public on Jul 12, 2006
Title EGF 4h replicate 1
Sample type RNA
 
Source name optic nerve head astrocyte
Organism Rattus norvegicus
Characteristics primary cell culture
Extracted molecule total RNA
Extraction protocol Primary astrocyte cultures from optic nerve head. Cells were lysed in separate groups in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 3 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description control astrocytes
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Sep 20, 2005
Last update date Jul 11, 2006
Contact name Bin Liu
E-mail(s) liu@vision.wustl.edu
Phone 314-747-4183
Fax 314-747-4211
Organization name Washington University
Department Ophthalmology
Lab Neufeld
Street address 660 S. Euclid
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL1355
Series (1)
GSE5282 Astrocytes in response to activation of EGFR

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
1367452_at 22866.6 P
1367453_at 12933.1 P
1367454_at 13152.8 P
1367455_at 22381.5 P
1367456_at 29495.6 P
1367457_at 6507.2 P
1367458_at 2351.6 P
1367459_at 33199.4 P
1367460_at 31412.5 P
1367461_at 12606.3 P
1367462_at 11971.9 P
1367463_at 11281.5 P
1367464_at 5875.3 P
1367465_at 10024.9 P
1367466_at 7986.7 P
1367467_at 8977.6 P
1367468_at 2794.4 P
1367469_at 44098.8 P
1367470_at 23566.7 P
1367471_at 4630.1 P

Total number of rows: 31099

Table truncated, full table size 576 Kbytes.




Supplementary file Size Download File type/resource
GSM75265.CEL.gz 4.2 Mb (ftp)(http) CEL

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