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Sample GSM753444 Query DataSets for GSM753444
Status Public on Apr 19, 2017
Title Reference Epigenome: ChIP-Seq Analysis of H3K9me3 in BMP4 Mesendoderm Cells; renlab.H3K9me3.BMP4M.02.01
Sample type SRA
Source name Human embryonic stem cells received from the James Thomson laboratory. Cells are from the H1 line and are differentiated into mesendoderm by treatment with BMP4.; renlab.H3K9me3.BMP4M.02.01
Organism Homo sapiens
Characteristics sample alias: BMP4-04
sample common name: H1 BMP4 Derived Mesendoderm Cultured Cells
molecule: genomic DNA
disease: None
biomaterial_provider: James Thomson Laboratory
biomaterial_type: Cell Line
line: H1
lineage: Embryonic Stem Cell
differentiation_stage: Embryonic stem cell differentiated into mesendoderm by treatment with BMP4
differentiation_method: H1 cells in E8 medium contatin 5ng/mL BMP4 and 25ng/mL Activin A for 2 days
passage: Between 20 and 30
medium: E8
Sex: Male
batch: BMP4-4
experiment_type: Histone H3K9me3
extraction_protocol: See
extraction_protocol_type_of_sonicator: Biorupter
extraction_protocol_sonication_cycles: 80
chip_protocol: See
chip_protocol_chromatin_amount: 500 micrograms
chip_protocol_bead_type: magnetic anti-rabbit
chip_protocol_bead_amount: 33,500,000
chip_protocol_antibody_amount: 5 micrograms
chip_antibody: H3K9me3
chip_antibody_provider: Abcam
chip_antibody_catalog: ab8898
chip_antibody_lot: 699671
Extracted molecule genomic DNA
Extraction protocol Library construction protocol: See
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
Description sample_term_id: NTR_0000856
assay_term_id: OBI_0000716
Design description: ChIP-Seq Analysis of H3K9me3 in BMP4 Mesendoderm Cells. Sequencing was done on the Illumina Genome Analyzer IIx platform.
Library name: SK526
EDACC Genboree Experiment Page:
EDACC Genboree Sample Page:
For data usage terms and conditions, please refer to:
Data processing Various levels of processed data files will be made available as this project proceeds.
Submission date Jul 01, 2011
Last update date Apr 19, 2017
Contact name UCSD AND SALK
Organization name University of California, San Diego
Street address Health Sciences Drive
City La Jolla
State/province CA
ZIP/Postal code 92092
Country USA
Platform ID GPL10999
Series (1)
GSE16256 UCSD Human Reference Epigenome Mapping Project
BioSample SAMN00631731

Supplementary data files not provided
Raw data not applicable for this record
Processed data not provided for this record
Raw data not provided for this record

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