 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 19, 2024 |
| Title |
BLISS_shCDK12_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
U2OS
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U2OS
treatment: shCDK12
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were fixed with 2%PFA and processed according to the publised sBLISS protocol (Bouwman, B.A.M., Agostini, F., Garnerone, S. et al. Genome-wide detection of DNA double-strand breaks by in-suspension BLISS. Nat Protoc 15, 3894–3941 (2020). https://doi.org/10.1038/s41596-020-0397-2)
Libraries were prepared according to the published sBLISS-seq protocol
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Data were analysed according to the sBLISS-seq protocol (Bouwman, B.A.M., Agostini, F., Garnerone, S. et al. Genome-wide detection of DNA double-strand breaks by in-suspension BLISS. Nat Protoc 15, 3894–3941 (2020). https://doi.org/10.1038/s41596-020-0397-2)
Assembly: hg19
Library strategy: SBLISS-seq
|
| |
|
| Submission date |
Jul 05, 2023 |
| Last update date |
Jun 19, 2024 |
| Contact name |
Stefano Campaner |
| E-mail(s) |
stefano.campaner@iit.it
|
| Organization name |
fondazione Istituto italiano di tecnologia
|
| Department |
Center for Genomic Science
|
| Lab |
Cancer Biology
|
| Street address |
Via adamello 16
|
| City |
Milano |
| ZIP/Postal code |
20139 |
| Country |
Italy |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE236547 |
CDK12 prevents MYC-induced transcription-replication conflicts [BLISS] |
| GSE236553 |
CDK12 prevents MYC-induced transcription-replication conflicts |
|
| Relations |
| BioSample |
SAMN36313361 |
| SRA |
SRX20904022 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM7549770_BLISS_shCDK12_rep1.bw |
1.1 Mb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
