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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 19, 2024 |
| Title |
BLISS_shCDK12_rep2 |
| Sample type |
SRA |
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| Source name |
U2OS
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| Organism |
Homo sapiens |
| Characteristics |
cell line: U2OS
treatment: shCDK12
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were fixed with 2%PFA and processed according to the publised sBLISS protocol (Bouwman, B.A.M., Agostini, F., Garnerone, S. et al. Genome-wide detection of DNA double-strand breaks by in-suspension BLISS. Nat Protoc 15, 3894–3941 (2020). https://doi.org/10.1038/s41596-020-0397-2)
Libraries were prepared according to the published sBLISS-seq protocol
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| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Data were analysed according to the sBLISS-seq protocol (Bouwman, B.A.M., Agostini, F., Garnerone, S. et al. Genome-wide detection of DNA double-strand breaks by in-suspension BLISS. Nat Protoc 15, 3894–3941 (2020). https://doi.org/10.1038/s41596-020-0397-2)
Assembly: hg19
Library strategy: SBLISS-seq
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| Submission date |
Jul 05, 2023 |
| Last update date |
Jun 19, 2024 |
| Contact name |
Stefano Campaner |
| E-mail(s) |
stefano.campaner@iit.it
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| Organization name |
fondazione Istituto italiano di tecnologia
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| Department |
Center for Genomic Science
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| Lab |
Cancer Biology
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| Street address |
Via adamello 16
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| City |
Milano |
| ZIP/Postal code |
20139 |
| Country |
Italy |
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| Platform ID |
GPL24676 |
| Series (2) |
| GSE236547 |
CDK12 prevents MYC-induced transcription-replication conflicts [BLISS] |
| GSE236553 |
CDK12 prevents MYC-induced transcription-replication conflicts |
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| Relations |
| BioSample |
SAMN36313360 |
| SRA |
SRX20904023 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM7549771_BLISS_shCDK12_rep2.bw |
747.1 Kb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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