|
| Status |
Public on Jul 01, 2012 |
| Title |
glioma 105 |
| Sample type |
RNA |
| |
|
| Source name |
FFPE glioma tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: brain
cell type: glioma
histological diagnosis: glioblastoma
grade: 4
survival (yrs): 1.03
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Five sections of 10 µm thick were cut from each tissue block. The High Pure RNA Paraffin Kit (Roche Applied Science, Mannheim, Germany) was used to isolate the RNA from the paraffin.
|
| Label |
Biotin
|
| Label protocol |
Sample labeling was performed by AROS Applied Biotechnology AS (Arhus, Denmark) according to standard Affymetrix protocols in combination with Nugen WT-Ovation technology (FFPE V2 and Exon modules) (San Carlos, CA, USA) (n=55).
|
| |
|
| Hybridization protocol |
Array hybridization was performed by AROS Applied Biotechnology AS (Arhus, Denmark) according to standard Affymetrix protocols in combination with Nugen WT-Ovation technology (FFPE V2 and Exon modules) (San Carlos, CA, USA) (n=55).
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
A933-06.CEL
|
| Data processing |
Data were processed using GeneBASE software. MAT background correction were applied to probe intensities, gene level expression were summarized from the corrected intensities and then quantile normalized.
probe group file: HuEx-1_0-st-v2.r2.pgf
|
| |
|
| Submission date |
Jul 07, 2011 |
| Last update date |
Jul 01, 2012 |
| Contact name |
Lonneke AM Gravendeel |
| E-mail(s) |
a.gravendeel@erasmusmc.nl
|
| Phone |
0031107044333
|
| Fax |
0031107044365
|
| Organization name |
Erasmus MC
|
| Department |
Neurology
|
| Lab |
Neuro-oncology
|
| Street address |
Dr Molewaterplein 50
|
| City |
Rotterdam |
| State/province |
South Holland |
| ZIP/Postal code |
3000 CA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL5188 |
| Series (1) |
| GSE30472 |
Gene expression profiles of gliomas in formalin-fixed paraffin-embedded material |
|
